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Instant Enzymes
from Carolina Biotechnology 

Restriction enzymes take their names from the bacteria that produce them. EcoRI (the first such enzyme to be discovered) comes from Escherichia coli, strain RY13. The numeral I in the enzyme's name indicates that this was the first restriction enzyme found in E. coli. BamHI is obtained from Bacillus amyloliquefaciens H, (with the numeral I again showing that this was the first restriction enzyme found in this bacterium). HindIII is made by Haemophilus influenzae Rd. Here the III indicates that this was the third restriction enzyme isolated from H. influenzae. Many restriction enzymes are now obtained from high-yielding strains of bacteria that have been modified genetically. Traditionally, restriction enzymes have required careful handling and storage in a nonfrost-free freezer. This has been inconvenient for some users.

The latest development in restriction enzyme technology is room-temperature storage of restriction enzymes. No longer do you have to worry about enzymes losing activity! These enzymes are freeze-dried in reaction buffer as well, so you don't have to add the buffer separately. And, you won't have to aliquot the restriction enzyme since they come in individual reaction tubes. So there's no more waste from pipetting errors. Each tube comes with sufficient enzyme activity to digest 1.5 µg of DNA in 20 min. Packaged in strips of 8 or 16 tubes.

 W1-21-1720  BamHI  8 tubes  $12.00
 W1-21-1721  16 tubes  $23.00
 W1-21-1722 EcoRI  8 tubes  $12.00
 W1-21-1723  16 tubes  $23.00
 W1-21-1724  HindIII  8 tubes  $12.00
 W1-21-1725  16 tubes  $23.00

From LifeTechnologies, Carolina's partner in bringing biotechnology to your classroom.

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To order Biotechnology materials from Carolina
Call: 1-800-334-5551
Fax: 1-800-222-7112
For Technical Help Call: 1-800-227-1150

Copyright 1998 by Carolina Biological Supply Co.

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